Characterization of malate metabolism enzymes during seed filling

COSTA, JOQUÍN; BADIA, MARIANA BEATRIZ; PAVLOVIC, TATIANA; ANDREO, CARLOS SANTIAGO; DRINCOVICH, MARÍA FABIANA; SAIGO, MARIANA; GERRARD WHEELER, MARIEL CLAUDIA

Congreso; Reunión conjunta de sociedades de biociencias; 2017

Seeds are of great economic interest due to the value of their organic composition. The metabolic pathways leading to the deposition of triacylglycerols and proteins in seeds are complex in terms of the amount of enzymes involved, regulatory context and variability between species. Previous studies of our group indicated that NAD- and NADP-dependent malic enzymes (ME) participate in the provision of reducing power and citrate or acetyl-CoA, respectively, to the pathways of reserve biosynthesis during soybean maturation. In this context, the aim of this work was to analyze the biochemical and regulatory properties of the enzymes belonging to this family. For this, the different ME isoforms were obtained through their cloning, heterologous expression in Escherichia coli and subsequent purification