Non-photosynthetic ?malic enzyme? from maize: a constituvely expressed enzyme that responds to plant defence inducers

VERÓNICA MAURINO; MARIANA SAIGO; CARLOS ANDREO; MARÍA F. DRINCOVICH

PLANT MOLECULAR BIOLOGY

Springer Netherlands

Año: 2001 vol. 45 p. 409 - 420

0167-4412

The characterization of a non-photosynthetic isoform of NADP - malic enzyme (NADP-ME) from maize roots, which represents nearly 7% of the total soluble protein of this tissue, was performed. The molecular properties of the purified protein, as well as the kinetic parameters determined, indicate that the NADP-ME isoform present in maize roots differs from the photosynthetic enzyme implicated in the C4 cycle, but is similar, or identical, to the enzyme previously characterized from etiolated maize leaves (Maurino, Drincovich and Andreo, Biochem. Mol. Biol. Int. 38 (1996) 239?250). A full-length ORF encoding a plastidic NADP-ME (almost identical to the maize root NADP-ME, GenBank accession number U39958) was cloned from a root cDNA library as well as isolated by reverse transcription (RT)-PCR using green leaves mRNA as template. These results indicate that root NADPME does not constitute a root-specific isoform, but represents a protein with a constitutive pattern of expression in plastids of the C4 plant maize. The amount of NADP-ME measured by activity, western and northern blot was modified when different stress conditions (including treatments with cellulase, fungal elicitors, jasmonate and hypoxic treatment)were applied to maize roots, indicating that the enzyme frommaize roots is under transcriptional or post-transcriptional regulation by effectors related to plant defence responses. It is deduced that the induction of housekeeping genes, like non-photosyntheticNADP-ME, whose constitutive role may be the provision of reductivepower in non-photosynthetic plastids, is likely to accompany the defence response.