Inhibition of DHEA production by factors released from M. tuberculosis-stimulated peripheral blood mononuclear cells is abrogated by transforming growth factor-beta; neutralization

DATTILIO L; BOZZA V; SANTUCCI N; BONGIOVANNI B; DIDOLI G; RADCLIFFE S; BESEDOVSKY H; DEL REY A; BOTTASSO O; BAY ML

ANNALS OF THE NEW YORK ACADEMY OF SCIENCES.

BLACKWELL PUBLISHING

Lugar: New York; Año: 2012 vol. 1262 p. 1 - 9

0077-8923

Supernatants (SN) from cultures of peripheral blood mononuclear cells (PBMC) of tuberculosis patients (TB) inhibit dehydroepiandrosterone (DHEA) secretion by the adrenal cell line NCI-H295R. To analyze whether TGF-B is involved in this effect, SN of PBMC from healthy controls (HCo) or severe TB, stimulated or not with M. tuberculosis (SNMtb) during 36 hs, were added to adrenal cells under basal conditions or following stimulations with Forskolin. Cortisol and DHEA concentrations were evaluated in supernatants of the adrenal cells cultured for 48 h without or with addition of anti-TGF-B. Treatment with SNMtb-TB inhibited DHEA production and this effect was reversed when SN were treated with anti-TGF-B. The increased in cortisol production was not affected by anti-TGF-B treatment. In conclusion, mediators released during the anti-tuberculous immune response differentially modulate steroid production by adrenal cells, and TGF-B is an important cytokine implied in the inhibition of DHEA production observed in TB.