StCDPK1 transgenic potato plants display an anomalous response to gibberellins

GARGANTINI P; GIAMMARIA V; ACCORINTI J; ULLOA RM

Iguazú, Misiones

Congreso; XL reunión de SAIB; 2004

Sociedad Argebtina de Investigaciones Bioquímicas

Potato tuberization is a complex organogenic process in which an underground stolon develops into a storage organ. During stolon to tuber transition different calcium dependent protein kinases (StCDPKs) are differentially expressed. StCDPK1 expression is enhanced in developing stolons and is induced by sucrose suggesting that it could be involved in the signalling cascade triggered upon tuberization. Transgenic potato plants that displayed different StCDPK1 expression (0,1X to 4X) than the wild type plants were produced. Stem cuttings of the different transgenic lines were grown in tuber inducing conditions (MS with 8% sucrose) with the addition of GAs (0,05 and 0, 5uM) or of the GAs inhibitor, CCC (0,05 ?0,5 mg/ml). B7 plants with reduced StCDPK1 expression didn?t respond to GAs (0,05 amd 0, 5) while the other transgenic lines showed an anomalous dose-response pattern. In addition, B7 plants developed tubers in MS 8% earlier than the control plants.