itric oxide (NO) but not TNF alpha inhibits germ cell cycle progression and impairs rat spermatogenesis

FERREIRO, MARIA EUGENIA; MENDEZ, CINTHIA SOLEDAD; LEILANE GLIENKE; SOBARZO CRISTIAN MARCELO,; FERRARI,S MARÍA JIMENA; JACOBO PV,; THEAS MARIA SUSANA

Congreso; REUNION ANUAL DE LA SOCIEDAD DE INVESTOGACION CLINICA; 2021

Low spermatogenic efficiency in infertile men is not only due to post meiotic events, but also to decreased meiotic activity and spermatogonia (Spg) number. We demonstrated that Spg decreased number negatively correlates with the number of immune cells in testicular biopsies of azoospermic patients. Proinflammatory agents nitric oxide (NO) and TNFα produced by the immune cells that infiltrate the testis might impair spermatogenesis. Our objective was to evaluate the effect of NO and TNFα on Spg and preleptotene spermatocyte (PLs) proliferation and spermatogenesis progression in adult Wistar rats. DETA-NOnoate (DETA-NO), a NO donor, or TNFα were injected in one testis, saline was injected in the contralateral testis. On day 5, a group of animals received BrdU injection (ip) and were euthanized 2h later in order to evaluate proliferation, by immunofluorescense. Another group was sacrificed on day 60 to evaluate the effect on spermatogenesis. DETA-NO (2 and 10mM) significantly reduced the number of BrdU+Spg/seminiferous tubule (ST) (p