Evaluation of proliferation and maturation markers of Sertoli cells in the testes of infertile patients.

LEILANE GLIENKE; MAIO MARIA BELEN; MUNDURUCA PIRES THAISY; ROJAS CAMPIÓN IGNACIO; HERNAN OXILIA; BARBERIS MIRTA; FULCO, MARÍA FLORENCIA; BERENSZTEIN EPSERANZA; LUSTIG, LIVIA; SCIURANO ROBERTA; SOBARZO CRISTIAN MARCELO,; THEAS MARÌA SUSANA

Congreso; LVII Reunión Anual SAIC 2022; 2022

SAIC, SAFIS, SAI

Testis immunopathology is similar in humans and rats undergoing chronic testicular inflammation and infertility. We showed that the number of Sertoli cells (SCs) per seminiferous tubules (STs) increases in testicular biopsies of patients, who underwent assisted fertilization procedure, displaying severe hypospermatogenesis (HypoS) and Sertoli cell only syndrome (SCOS); as well as in rats with autoimmune orchitis (EAO). EAO is a well-established rodent model of organ-specific autoimmunity associated with infertility. To verify if the increased number of SCs is associated with their maturity degree we analyzed by immunohistochemistry the expression of anti-Müllerian hormone (AMH), Ki67, and serum FSH (RIA). STs wall thickness was also evaluated.In severe HypoS (n=3-5) and SCOS (n=3-5) patients the percentage of STs with AMH+ SCs significantly increased compare to patients with obstructive azoospermia and complete spermatogenesis (control group, C, n=3-6) (mean±SEM, C: 0,00±0,00; severe HypoS: 86,78±5,18; SCOS: 94,23±5,28; p