INVESTIGADORES
STRUMIA Miriam Cristina
artículos
Título:
Dendritic polyglycerolamine as a functional antifouling coating of gold surfaces.
Autor/es:
JULIETA PAEZ; VERÓNICA BRUNETTI; TOBIAS BECHERER; TIHOMIR SOLOMUN; MIRIAM STRUMIA; TIHOMIR SOLOMUN; JORGE MIGUEL; MARCELO CALDER; RAINER HAAG
Revista:
JOURNAL OF MATERIALS CHEMISTRY
Editorial:
ROYAL SOC CHEMISTRY
Referencias:
Lugar: CAMBRIDGE; Año: 2012 vol. 22 p. 19488 - 19497
ISSN:
0959-9428
Resumen:
Dendritic polyglycerol (PG) functionalized surfaces represent a good alternative for preparation of
protein resistant materials, whose versatility can be enhanced by conferring them the ability to bind
particular biomolecules of interest to the surface. In this work, PG derivatives bearing disulfide and
different loadings of amino moieties (0?14%) were synthesized and attached to gold surfaces. The
modified surfaces were characterized by means of infrared reflection adsorption spectroscopy
(FT-IRRAS), X-ray photoelectron spectroscopy (XPS), and contact angle measurements. The protein
resistance properties of the PG-modified surfaces were evaluated by surface plasmon resonance (SPR)
spectroscopy using fibrinogen, albumin, pepsin, and lysozyme as model proteins. The availability and
accessibility of the amino groups to bind biomolecules were assessed by fluorescence measurements.
This study demonstrates that PG-coated surfaces with amino contents up to 9% still show very good
protein resistant properties. At the same time, the amino moieties on the surface are available and
reactive for selective ligand attachment. By fluorescence labeled DNA hybridization, the high
selectivity of these functional surfaces could be demonstrated.