Characterization of E3 ubiquitin ligase HERC1 as an important regulator of tumor cell invasion and migration

FABIANA ALEJANDRA ROSSI; MARIO ROSSI; JULIANA H. ENRIQUE´ STEINBERG; EZEQUIEL CALVO-ROITBERG; JOAQUIN M ESPINOSA

Simposio; Frontiers in Bioscience III; 2018

The invasive potential of malignant cells is characterized by a series of specific molecular and genetic alterations that allow them to penetrate the extracellular matrix and spread in the surrounding tissues. These changes are associated with alterations in a wide variety of posttranslational modification patterns, including the Ubiquitin-Proteasome System (UPS). The UPS plays a fundamental role in almost every single cellular process, and therefore it is not surprising that genetic alterations, abnormal expression or dysfunction of different components of this cascade are often associated with malignant transformation of tumor cells and the development of metastatic processes.Our results showed that Herc1 silencing reduces the migratory/invasive potential of MDA-MB-231 triple negative breast cancer (TNBC) cell line, both in vitro and more importantly in vivo. We aim to repeat this phenotypic characterization in other TNBC cell lines and obtain more information about Herc1 molecular mechanism of action