MANGANESE-EXPOSED BV-2 MICROGLIA INDUCES DAMAGE IN N27 DOPAMINERGIC NEURONAL CELLS

A ABUISO; ALCON, SOLEDAD PORTE; VINUESA A; FLAVIA EUGENIA SARAVIA; KOTLER, M; GOROJOD R

Congreso; Reunion anual de la Sociedad Argentina de Investigacion Clinica; 2021

SAIC

Manganese (Mn) intake is essential at physiological concentrations.However, prolonged exposure produces a neurodegenerative diseasecalled manganism, whose symptoms are often confused withidiopathic Parkinson´s disease. Even though the harmful effect ofMn in different cell types has been described, much remains to beunderstood regarding the outcomes of glial activation on neuronaldeath. Objective: To evaluate the effect of soluble mediators releasedby microglial cells after Mn exposure on neuronal integrity.For this purpose, we first characterized the direct effect of Mn exposureon neuronal and microglial cells, and then explored the influenceof microglia-conditioned medium (MCM) on neurons viability.Methodology: MCMs were generated by BV-2 cells incubation with250-1000 μM Mn for periods of 3 or 6 h, and were used to stimulateN27 neuronal cells for 24h; cell viability was assessed using MTTreduction assay; the study of ROS production was performed usingDCFDA; the change in mRNA expression was quantified by RT-qPCR.Results: In N27 neuronal cells, Mn exposure induced a concentration-dependent decrease in cell viability after 24h (100-1000 μM,P