INVESTIGADORES
SANCHEZ Sara Serafina Del V.
artículos
Título:
Heterologous Gap Junctions Between Oocyte and Follicle Cells in Bufo arenarum: Hormonal Effects on Their Permeability and Potential Role in Meiotic Arrest
Autor/es:
VILLECCO, E.I.; AYBAR M.J.; SANCHEZ RIERA A.; SANCHEZ S.S.
Revista:
ZYGOTE
Editorial:
CAMBRIDGE UNIV PRESS
Referencias:
Año: 1996 vol. 276 p. 76 - 85
ISSN:
0967-1994
Resumen:
The physiological significance of follicle cell-oocyte
gap junctional coupling in the ovarian follicle Bufo arenarum was
investigated. Lucifer Yellow injected into the oocyte readily passed to the
overlying follicle cells, demonstrating effective dye coupling. We determined that
the gap junction coupling varies during the annual sexual cycle. In winter
there is a marked decrease in the amount or absence of heterologous gap
junction contacts. In contrast, during the breeding season, gap junctions were
normally coupled. We studied in vivo and in vitro the effects of frog pituitary
hormone (FPH) and follicle stimulating hormone (FSH) on gap junction coupling.
The results suggest that FSH induce the coupling of gap junctions enabling a greater
metabolic cooperation necessary for oocyte growth and maintenance. On the other
hand, nuclear maturation under physiological conditions seemed to have a direct
consequence on the gap junction uncoupling and the interruption of the gap
junctional flow of an oocyte maturation inhibiti 3n factor by the effects of
LH-like gonadotropin or progesterone.