Genetic diversity of Fusarium graminearum sensu lato isolates from wheat associated with Fusarium Head Blight in diverse geographic locations of Argentina

V.F. CONSOLO; L.M. ORTEGA; G.L. SALERNO; A.L. ASTORECA; T.M. ALCONADA

REVISTA ARGENTINA DE MICROBIOLOGíA

Elsevier España

Lugar: Buenos Aires; Año: 2015 vol. 47 p. 245 - 250

0325-7541

Fusarium Head Blight is an important wheat disease in the Argentine Pampas region,being  Fusarium graminearum the predominant pathogen. DNA polymorphism of the isolates was analyzed by IGS-RFLP and ISSR. IGS-RFLP and ISSR profiling were carried out using six endonucleases  and eight primers, respectively. IGS-RFLP yielded 41 bands, 30 of which werepolymorphic  while ISSR produced 87 bands with 47 polymorphic bands. Both markers showedgenetic  variability among the analyzed isolates; however, IGS-RFLP was more efficient thanISSR,  showing a higher polymorphic average (59.91%) than the latter (44.11%). The averages ofpolymorphic  information content (PIC) were 0.211 and 0.129, respectively. Twenty haplotypeswere  identified by IGS-RFLP and 15 haplotypes by ISSR. Genotype clustering within dendrograms was  different for both types of markers. The genetic groups obtained by IGS-RFLP showed a  partial association to geographic origin. This is the first report on genetic variability of F.graminearum   isolates  from wheat in Argentina using IGS-RFLP and ISSR markers.