Microcystis aeruginosa strain [D-Leu1] Mcyst-LR producer, from Buenos Aires province, Argentina

L. ROSSO; D. SEDÁN; M.A KOLMAN; J. CAIXACH; J.M. OTEIZA; G.L. SALERNO; R. ECHENIQUE; L. GIANNUZZI; D. ANDRINOLO

Journal of Coastal Life Medicine

Haikou Yongfa Printing Co., Ltd

Lugar: ShunHai Qu, Haikou; Año: 2014 vol. 2 p. 287 - 296

2309-5288

Objective: To show the toxicological and phylogenetic characterization of a native Microcystisaeruginosa (M. aeruginosa) strain (named CAAT  2005-3)  isolated from a water body of Buenos Aires province, Argentine.Methods: A M. aeruginosa strain was isolated from the drainage canal of the sewage treatment inthe town of Pila, Buenos Aires province, Argentina and acclimated to laboratory conditions. Theamplification of cpcBAIGS Phcocyanin (PC, intergenic spacer and flanking regions) was carriedout in order to build a phylogenetic tree. An exactive/orbitrap mass spectrometer equipped withan electrospray ionization source (Thermo Fisher Scientific, Bremen, Germany) was used for the LC/ESIHRMS microcystins analysis. The number of cell/mL and [D-Leu] Mcyst-LR productionobtained as a function of time was modelled using the Gompertz equation.Results:The phylogenetic analysis showed that the sequence clustered with others M. aeruginosasequences obtained from NCBI . The first Argentinian strain of M. aeruginosa (CAAT  2005-3)growing under culture conditions maintains the typical colonial architecture of M. aeruginosa with profuse mucilage. M. aeruginosa CAAT  2005-3 expresses a toxin variant, that was identifiedby LC HRMS / O rbitrapas as [D-Leu ] microcystin-LR ([M + H] + =1037.8 m/z). Conclusions: [D-Leu111] microcystin-LR has been also detected in M. aeruginosa samples from Canada, Brazil and Argentina. This work provides the basis for technological development andproduction of analytical standards of toxins present in our region.