INVESTIGADORES
SALERNO Graciela Lidia
artículos
Título:
Sucrose is involved in diazotrophic metabolism of the heterocyst-forming cyanobacterium Anabaena sp
Autor/es:
L. CURATTI; E. FLORES; G.L. SALERNO
Revista:
FEBS LETTERS
Editorial:
Elsevier Science Ltd
Referencias:
Lugar: Amsterdam, The Netherlands; Año: 2002 vol. 513 p. 175 - 178
ISSN:
0014-5793
Resumen:
Sucrose synthase (SuS) expression was studied in thefilamentous, nitrogen-fixing cyanobacterium Anabaena sp. PCC7119. SuS activity, SusA polypeptide, and susA mRNA levelswere lower in cells cultured diazotrophically than in the presenceof combined nitrogen. An insertional susA mutant presented adramatic increase in sucrose levels, whereas the disaccharidewas not detectable in a susA overexpressing strain, indicatingthat SusA is involved in the cleavage of sucrose in vivo.Diazotrophic growth was impaired in the susA overexpressingstrain, suggesting a role for sucrose in diazotrophic metabolismand the involvement of SusA in the control of carbon flux in theN2-fixing filament. ß 2002 Published by Elsevier Science B.V.on behalf of the Federation of European Biochemical Societies
