INVESTIGADORES
SALERNO Graciela Lidia
artículos
Título:
First evidence of sucrose biosynthesis by single cyanobacterial bimodular proteins
Autor/es:
G.M.A. MARTÍNEZ-NOËL; A.C. CUMINO; M.A. KOLMAN; G.L. SALERNO
Revista:
FEBS LETTERS
Editorial:
ELSEVIER SCIENCE BV
Referencias:
Lugar: Amsterdam; Año: 2013 vol. 587 p. 1669 - 1674
ISSN:
0014-5793
Resumen:
The net synthesis of sucrose (Suc) is catalysed by the sequential action of Suc-phosphate synthase (SPS)
and Suc-phosphate phosphatase (SPP). SPS and SPP from Anabaena sp. PCC 7120 (7120-SPS and 7120-SPP) define
minimal catalytic units. Bidomainal SPSs, where both units are fused, occur in
plants and cyanobacteria, but they display only SPS activity. Using recombinant proteins
that have fused 7120-SPS and 7120-SPP, we demonstrated that they are
bifunctional chimeras and that the arrangement 7120-SPS/SPP is the most
efficient to catalyse the sequential reactions to yield Suc. Moreover, we
present the first evidence of a bidomainal SPS present in the cyanobacterium Synechococcus elongatus PCC 7942 with
both, SPS and SPP activity.