INVESTIGADORES
SALERNO Graciela Lidia
artículos
Título:
Sucrose may play a different role other than osmoprotectant in Synechocystis sp. PCC 6803 salt-shock cells
Autor/es:
DESPLATS, P.; FOLCO, E.; SALERNO, G.L.
Revista:
PLANT PHYSIOLOGY AND BIOCHEMISTRY
Editorial:
Elsevier
Referencias:
Año: 2005 vol. 43 p. 133 - 138
ISSN:
0981-9428
Resumen:
The role of sucrose in cyanobacteria is still not fully understood. It is generally considered a salt-response molecule, and particularly, in Synechocystis sp. strain PCC 6803, it is referred as a secondary osmolyte. We showed that sucrose accumulates transiently in Synechocystis cells at early stages of a salt shock, which could be ascribed to salt activation of sucrose-phosphate synthase (SPS, UDP-glucose: D-fructose-6-phosphate 2-a-D-glucosyltransferase; EC 2.4.1.14), the key enzyme in sucrose synthesis pathway, and to an increase of the expression of the SPS encoding gene. Experiments with a mutant strain impaired in sucrose biosynthesis showed that sucrose is essential in stationary-phase cells to overcome a later salt stress. Taken together, these results led us to suggest a more intricate function for sucrose than to be an osmoprotectant compound.
