Phylogenetic relationships of Fusarium poae based on EF-1a and mtSSU sequences

S.A. STENGLEIN; M.S. RODRIGUERO; E. CHANDLER; P. JENNINGS; G. L. SALERNO; P. NICHOLSON

Fungal Biology (ex-Mycological Research)

Elsevier

Año: 2009 vol. 114 p. 96 - 106

0953-7562

A molecular phylogenetic analysis of Fusarium poae isolates from South America(Argentina) and Europe (mainly England, Germany, Italy) was performed using 98 F. poae,four Fusarium culmorum, two Fusarium sporotrichioides and one Fusarium langsethiae isolates.Phylogenetic analyses were performed using nuclear (translation elongation factor 1-alpha,EF-1a) and mitochondrial (mitochondrial small subunit rDNA, mtSSU ) sequences.Partitioned (each dataset separately) and combined (EF-1a þ mtSSU ) analyses did notreveal any clear correlations from the inferred branching topology, between the distributionof observed haplotypes and the geographic origin and/or host species. Resultsfrom the present study confirmed that isolates from F. poae form a monophyleticgroup, and the low variability within isolates from a broad geographic range suggestsa common lineage history. Among F. poae isolates from Argentina, however, somewere found to possess an insert within mtSSU with structural similarities to groupIC2 introns. F. poae isolates differing by the presence/absence of a mtSSU insertionwere characterized further by analysis of a portion of the Tri5 gene, but this sequencewas unable to reveal variability. The presence of this insert only within isolates fromArgentina suggests that evolutionary events (insertions/deletions) are probably takingplace within the Argentinian F. poae isolates, and that the acquisition of this insertoccurred after geographic isolation of the Argentinian and European populations.