INVESTIGADORES
SALERNO Graciela Lidia
artículos
Título:
Sucrose metabolism: Anabaena sucrose-phosphate synthase and sucrose-phosphate phosphatase define minimal functional domains shuffled during evolution
Autor/es:
A.C. CUMINO; L. CURATTI; L.E. GIARROCCO; G.L. SALERNO
Revista:
FEBS LETTERS
Editorial:
Elsevier Science Ltd
Referencias:
Lugar: Amsterdam, The Netherlands; Año: 2002 vol. 517 p. 19 - 23
ISSN:
0014-5793
Resumen:
Based on the functional characterization of sucrosebiosynthesis related proteins [SBP: sucrose-phosphate synthase(SPS), sucrose-phosphate phosphatase (SPP), and sucrosesynthase (SuS)] in Anabaena sp. PCC7120 and sequenceanalysis, we have shown that SBP are restricted to cyanobacteriumspecies and plants, and that they are multidomainproteins with modular architecture. Anabaena SPS, a minimalcatalytic SPS unit, defines a glucosyltransferase domain presentin all SPSs and SuSs. Similarly, Anabaena SPP defines aphosphohydrolase domain characteristic of all SPPs and someSPSs. Phylogenetic analysis points towards the evolution ofmodern cyanobacterial and plant SBP from a bidomainalcommon ancestral SPS-like gene. ß 2002 Federation of EuropeanBiochemical Societies. Published by Elsevier Science B.V.All rights reserved
