THYMIC STROMAL LYMPHOPOIETIN (TSLP) IS A NEUTROPHIL CELLS MODULATOR: RELEVANCE IN THE PATHOGENESIS OF BRAIN TUMORS

CORONEL JV, ; ITURRIZAGA J, ; INFANTE A, ; JANCIC C, ; VERMEULEN M, ; CANDOLFI M, ; VILLAVERDE M, ; RABADÁN A, ; LENICOV FR; SALAMONE G

Congreso; Reunión anual de Sociedades Bioscientíficas. SAI-SAIC-SAFIS; 2020

Thymic stromal lymphopoietin (TSLP) is a Neutrophil cells modulator: relevance in the pathogenesis of brain tumors.Juan Valentin Coronel 1, Juan Iturrizaga 1,2, Alejandra Infante 1, Carolina Jancic 1,3, Monica Vermeulen 1,3, Marianela Candolfi 5, Marcela Villaverde 6, Alejandra Rabadán 2, Federico Remes Lenicov 4, and Gabriela Salamone 1,3. 1 Instituto de Medicina Experimental (IMEX) CONICET ? Academia Nacional de Medicina, Buenos Aires, Argentina.2 División Neurocirugía del Instituto de Investigaciones Médicas A Lanari. Universidad de Buenos Aires. Universidad de Buenos Aires.3 Departamento de Microbiología, Parasitología e Inmunología, Facultad de Medicina, Universidad de Buenos Aires. Buenos Aires, Argentina. 4 Instituto de Investigaciones Biomédicas en Retrovirus y SIDA (INBIRS), Universidad de Buenos Aires ? CONICET, Argentina. 5 Instituto de Investigaciones Biomédicas (INBIOMED UBA-CONICET), Facultad de Medicina, UBA.6 Unidad de Transferencia Genética, Área Investigación, Inst. de Oncología Ángel H. Roffo, Facultad de Medicina, UBA.Background: Glioblastoma (GBM) is the most devastating brain tumor, with an associated poor prognosis. Despite the advances in surgery and chemoradiation, the survival of GBM patients has not improved significantly in the past three decades. Thymic stromal lymphopoietin (TSLP) is a cytokine produced primarily by activated epithelial cells. TSLP has been shown to be a key factor in maintaining immune homeostasis and regulating inflammatory responses at mucosal barriers, recent studies have found an expanding role for TSLP in inflammatory diseases and cancer. This work aimed to elucidate the relevance of TSLP in the interaction between neutrophils and GBM cells. First, we evaluated the production of TSLP by tumor cells through RT-PCR and then, we determined whether the TSLP treatment affects the cross-talk between neutrophils and tumor GBM cells. For those purposes human U251 cell line or tumoral cells obtained from a GBM patient were co-cultured with human neutrophils. We observed that the U251 cell line or the primary cell culture incubated with Epidermal Growth Factor (EGF) produced TSLP (p