MOUSE PATERNAL ALCOHOL EXPOSURE AFFECTS SPERMATOZOA AND COMPROMISES THE SURVIVAL OF ITS OFFSPRING

GOTFRYD L; G. SALAMONE; CAMBIASSO M,; STINSON MG; J.C.CALVO; FONTANA V.

Congreso; Reunión conjunta SAIC-SAI-SAFE; 2018

Previously we observed that male alcohol consumption delayed embryo differentiation by deregulation of peri-implantation events and alteration of embryo morphogenesis in vitro. Aim: To evaluate the effect of paternal alcohol consumption on spermatozoa, embryo and litter survival on a mouse model. Methods: CF-1 male mice were exposed (treated group, T) or not (control group, C) to 15% (v/v) ethanol in drinking water ad libitum for 15 days. Spermatozoa from cauda were obtained by swim-out to determine sperm concentration, motility, head decondensation (15, 30 and 60 min) and apoptosis by Annexin V-FITC on both groups. Control and treated males were mated with non-treated CF-1 females (1:1). Pregnant females (positive vaginal plug: day1) were sacrificed at day 2 of gestation to obtain 2-cell embryos which were cultured in vitro for 7 days and apoptosis was determined. Pregnancy outcomes were also evaluated and litter mortality, weight and size registered. Results: Male alcohol consumption did not alter the number of mated female but significantly increased sperm head decondensation compared to controls (p