CHOLINERGIC MODULATION OF POLYMORPHONUCLEAR AND MONOCYTES CELLS ACTIVATION

MOVERER LUCIANA*; GORI SOLEDAD*; VERMEULEN MONICA; SCORDO WALTER; JANCIC CAROLINA; SALAMONE GABRIELA

Buenos Aires

Congreso; . Reunión Anual Conjunta LXIII SAI - IV LASID ? II FAIC; 2015

Acetylcholine is an endogenous neurotransmitter of both the peripheral and central nervous systems where it is synthesised, stored, and released from cholinergic nerve terminals. However, there is now increasing evidence for extraneuronal acetylcholine signalling, which has been referred to as a ?non-neuronal cholinergic system?. The neutrophils and monocytes are importantcellular effectors associated with many pathofhysiologies and their cholinergic anti-inflammatory pathway is a potencial target to interrupt several diseases. Previously we describe the presence of muscarinic receptors (mAchR), choline acetyltransferase (ChAT) and acetylcholinesterase (AChE) in human myeloid dendritic cell (DC) and we also shown their cholinergic modulation. To furthercomplement our results the aim of this work is to analyze the cholinergic modulation of polymorphonuclear (PMN) and monocytes (Mo) cells. Methods: The PMN and Mo were isoleted from peripheral blood obtained from healthy nonsmoker. The PMN were cultured with or without Ach (10-7 to 10-10M) or FMLP 10-3M. Production of cytokines was determined by ELISA and the molecular expression by FACs. Results: We do not find differences in the release of IL-8 and TNF-ain monocytes cells, but we found significant differences when the cells were treated with carbachol 10-8M (P