ACETYLCHOLINE POLARIZES DENDRITIC CELLS TOWARD A TH2-PROMOTING PROFILE

MARÍA SOLEDAD GORI,; MÓNICA VERMEULEN, ; FEDERICO REMES-LENICOV, ; FLORENCIA SABBIONE, ; CAROLINA JANCIC; WALTER SCORDO, ; ANA CEBALLOS, ; NADIA TOWSTYKA, ; JORGE GEFFNER; GABRIELA SALAMONE

Congreso; Reunión Anual Conjunta LXIII SAI - IV LASID ? II FAIC; 2015

A growing body of research shows a reciprocal regulation between the neural and immune systems.Acetylcholine (ACh) is the most important parasympathetic neurotransmitter and increasing evidenceindicates that it is able to modulate the immune response. Interestingly, in recent years it has becomeclear that immune cells express a nonneuronal cholinergic system, which is stimulated in the course ofinflammatory processes. We have previously shown that dendritic cells (DC) express muscarinicreceptors, as well as the enzymes responsible for the synthesis and degradation of ACh. Here, weanalyzed whether ACh could also modulate the functional profile of DC. METHODSDendritic cells were obtained from monocytes cultured for 5 days with GM-CSF+IL-4. The phenotype ofDC was evaluated by flow cytometry and the production of cytokines analyzed by ELISA or intracellularstaining and flow cytometry.RESULTSTreatment of DC with ACh stimulated the expression of the Th2-promoter OX40L (p