VIP induces the decidualization program on human endometrial stromal cells and conditions dendritic cells profile

ESTEBAN GRASSO, ; SOLEDAD GORI; DANIEL PAPARINI, ; GABRIELA SALAMONE; GUSTAVO MARTINEZ, ; CLAUDIA PÉREZ LEIRÓS,; ROSANNA RAMHORST

Congreso; LXII de la Sociedad Argentina de Inmunología; 2014

The decidualization program involves phenotype and functional changes on endometrial cells that will facilitate the attachment and invasion of the blastocyst. This process involves the modulation of different mediators such as cytokines, chemokines and growth factors. Particularly, the vasoactive intestinal peptide (VIP) is a neuropeptide produced by endometrial stromal cells among others, and displays multiple target circuits that allow immunotolerance. Here, we investigated VIP contribution to the decidualization program and whether this process modulates dendritic cells (DC) profile. We differenciated human endometrial stromal cell line (hESC) in the presence of VIP (10-7M), or with medroxiprogesterone (MPA) and dbcAMP (Positive control) during 8 d. The decidualization performed with MPA+dbcAMP increased VIP expression (by RT-PCR) and secretion (by ELISA). When the decidualization was induced by VIP, we observed an increase IGFBP1, PRL and KLF13/KLF9 ratio in a concentration-dependent manner; accompanied by and increase in the expression of IL-8 and SDF1, both markers of receptive endometrium (p