“Preferential staining of activated CD8 T lymphocytes by mAbs 86.6”

M.C.SALAMONE, G.V.SALAMONE, G.B.REYES, M.BARBOZA, M.KADO,R.RAMHORST AND L.FAINBOIM

In Leukocyte Typing VI,

Garland Publishing, Inc. (A Member of the Taylor and Francis Group).

Año: 1997; p. 204 - 205

Human CD80, a member of the Ig superfarnily, was origindly described as a B-cell activation antigen. Subsequent studies have shown it also to be expressed on mature dendritic cells and activated T cells, although conflicting findings have been reported on the expression of CD86 by the latter cell type [ll. A modest increase in CD86 expression after three days of activation with phytohemagglutinin (PHA) was reported in the Fifth Workshop. The present work aimed to document the reactivity of the CD80, and CD86 monoclonal antibody (mAb) Panel on T-cell subsets at different times after PHA activation. at different times after PHA activation. at different times after PHA activation. at different times after PHA activation. (PHA) was reported in the Fifth Workshop. The present work aimed to document the reactivity of the CD80, and CD86 monoclonal antibody (mAb) Panel on T-cell subsets at different times after PHA activation. at different times after PHA activation. at different times after PHA activation. at different times after PHA activation. (PHA) was reported in the Fifth Workshop. The present work aimed to document the reactivity of the CD80, and CD86 monoclonal antibody (mAb) Panel on T-cell subsets at different times after PHA activation. at different times after PHA activation. at different times after PHA activation. at different times after PHA activation. (PHA) was reported in the Fifth Workshop. The present work aimed to document the reactivity of the CD80, and CD86 monoclonal antibody (mAb) Panel on T-cell subsets at different times after PHA activation. at different times after PHA activation. at different times after PHA activation. at different times after PHA activation. after three days of activation with phytohemagglutinin (PHA) was reported in the Fifth Workshop. The present work aimed to document the reactivity of the CD80, and CD86 monoclonal antibody (mAb) Panel on T-cell subsets at different times after PHA activation. at different times after PHA activation. at different times after PHA activation. at different times after PHA activation. (PHA) was reported in the Fifth Workshop. The present work aimed to document the reactivity of the CD80, and CD86 monoclonal antibody (mAb) Panel on T-cell subsets at different times after PHA activation. at different times after PHA activation. at different times after PHA activation. at different times after PHA activation. (PHA) was reported in the Fifth Workshop. The present work aimed to document the reactivity of the CD80, and CD86 monoclonal antibody (mAb) Panel on T-cell subsets at different times after PHA activation. at different times after PHA activation. at different times after PHA activation. at different times after PHA activation. (PHA) was reported in the Fifth Workshop. The present work aimed to document the reactivity of the CD80, and CD86 monoclonal antibody (mAb) Panel on T-cell subsets at different times after PHA activation. at different times after PHA activation. at different times after PHA activation. at different times after PHA activation. after three days of activation with phytohemagglutinin (PHA) was reported in the Fifth Workshop. The present work aimed to document the reactivity of the CD80, and CD86 monoclonal antibody (mAb) Panel on T-cell subsets at different times after PHA activation. at different times after PHA activation. at different times after PHA activation. at different times after PHA activation. (PHA) was reported in the Fifth Workshop. The present work aimed to document the reactivity of the CD80, and CD86 monoclonal antibody (mAb) Panel on T-cell subsets at different times after PHA activation. at different times after PHA activation. at different times after PHA activation. at different times after PHA activation. (PHA) was reported in the Fifth Workshop. The present work aimed to document the reactivity of the CD80, and CD86 monoclonal antibody (mAb) Panel on T-cell subsets at different times after PHA activation. at different times after PHA activation. at different times after PHA activation. at different times after PHA activation. (PHA) was reported in the Fifth Workshop. The present work aimed to document the reactivity of the CD80, and CD86 monoclonal antibody (mAb) Panel on T-cell subsets at different times after PHA activation. at different times after PHA activation. at different times after PHA activation. at different times after PHA activation. after three days of activation with phytohemagglutinin (PHA) was reported in the Fifth Workshop. The present work aimed to document the reactivity of the CD80, and CD86 monoclonal antibody (mAb) Panel on T-cell subsets at different times after PHA activation. at different times after PHA activation. at different times after PHA activation. at different times after PHA activation. (PHA) was reported in the Fifth Workshop. The present work aimed to document the reactivity of the CD80, and CD86 monoclonal antibody (mAb) Panel on T-cell subsets at different times after PHA activation. at different times after PHA activation. at different times after PHA activation. at different times after PHA activation. (PHA) was reported in the Fifth Workshop. The present work aimed to document the reactivity of the CD80, and CD86 monoclonal antibody (mAb) Panel on T-cell subsets at different times after PHA activation. at different times after PHA activation. at different times after PHA activation. at different times after PHA activation. (PHA) was reported in the Fifth Workshop. The present work aimed to document the reactivity of the CD80, and CD86 monoclonal antibody (mAb) Panel on T-cell subsets at different times after PHA activation. at different times after PHA activation. at different times after PHA activation. at different times after PHA activation. latter cell type [ll. A modest increase in CD86 expression after three days of activation with phytohemagglutinin (PHA) was reported in the Fifth Workshop. The present work aimed to document the reactivity of the CD80, and CD86 monoclonal antibody (mAb) Panel on T-cell subsets at different times after PHA activation. at different times after PHA activation. at different times after PHA activation. at different times after PHA activation. (PHA) was reported in the Fifth Workshop. The present work aimed to document the reactivity of the CD80, and CD86 monoclonal antibody (mAb) Panel on T-cell subsets at different times after PHA activation. at different times after PHA activation. at different times after PHA activation. at different times after PHA activation. (PHA) was reported in the Fifth Workshop. The present work aimed to document the reactivity of the CD80, and CD86 monoclonal antibody (mAb) Panel on T-cell subsets at different times after PHA activation. at different times after PHA activation. at different times after PHA activation. at different times after PHA activation. (PHA) was reported in the Fifth Workshop. The present work aimed to document the reactivity of the CD80, and CD86 monoclonal antibody (mAb) Panel on T-cell subsets at different times after PHA activation. at different times after PHA activation. at different times after PHA activation. at different times after PHA activation. after three days of activation with phytohemagglutinin (PHA) was reported in the Fifth Workshop. The present work aimed to document the reactivity of the CD80, and CD86 monoclonal antibody (mAb) Panel on T-cell subsets at different times after PHA activation. at different times after PHA activation. at different times after PHA activation. at different times after PHA activation. (PHA) was reported in the Fifth Workshop. The present work aimed to document the reactivity of the CD80, and CD86 monoclonal antibody (mAb) Panel on T-cell subsets at different times after PHA activation. at different times after PHA activation. at different times after PHA activation. at different times after PHA activation. (PHA) was reported in the Fifth Workshop. The present work aimed to document the reactivity of the CD80, and CD86 monoclonal antibody (mAb) Panel on T-cell subsets at different times after PHA activation. at different times after PHA activation. at different times after PHA activation. at different times after PHA activation. (PHA) was reported in the Fifth Workshop. The present work aimed to document the reactivity of the CD80, and CD86 monoclonal antibody (mAb) Panel on T-cell subsets at different times after PHA activation. at different times after PHA activation. at different times after PHA activation. at different times after PHA activation. after three days of activation with phytohemagglutinin (PHA) was reported in the Fifth Workshop. The present work aimed to document the reactivity of the CD80, and CD86 monoclonal antibody (mAb) Panel on T-cell subsets at different times after PHA activation. at different times after PHA activation. at different times after PHA activation. at different times after PHA activation. (PHA) was reported in the Fifth Workshop. The present work aimed to document the reactivity of the CD80, and CD86 monoclonal antibody (mAb) Panel on T-cell subsets at different times after PHA activation. at different times after PHA activation. at different times after PHA activation. at different times after PHA activation. (PHA) was reported in the Fifth Workshop. The present work aimed to document the reactivity of the CD80, and CD86 monoclonal antibody (mAb) Panel on T-cell subsets at different times after PHA activation. at different times after PHA activation. at different times after PHA activation. at different times after PHA activation. (PHA) was reported in the Fifth Workshop. The present work aimed to document the reactivity of the CD80, and CD86 monoclonal antibody (mAb) Panel on T-cell subsets at different times after PHA activation. at different times after PHA activation. at different times after PHA activation. at different times after PHA activation. after three days of activation with phytohemagglutinin (PHA) was reported in the Fifth Workshop. The present work aimed to document the reactivity of the CD80, and CD86 monoclonal antibody (mAb) Panel on T-cell subsets at different times after PHA activation. at different times after PHA activation. at different times after PHA activation. at different times after PHA activation. (PHA) was reported in the Fifth Workshop. The present work aimed to document the reactivity of the CD80, and CD86 monoclonal antibody (mAb) Panel on T-cell subsets at different times after PHA activation. at different times after PHA activation. at different times after PHA activation. at different times after PHA activation. (PHA) was reported in the Fifth Workshop. The present work aimed to document the reactivity of the CD80, and CD86 monoclonal antibody (mAb) Panel on T-cell subsets at different times after PHA activation. at different times after PHA activation. at different times after PHA activation. at different times after PHA activation. (PHA) was reported in the Fifth Workshop. The present work aimed to document the reactivity of the CD80, and CD86 monoclonal antibody (mAb) Panel on T-cell subsets at different times after PHA activation. at different times after PHA activation. at different times after PHA activation. at different times after PHA activation. latter cell type [ll. A modest increase in CD86 expression after three days of activation with phytohemagglutinin (PHA) was reported in the Fifth Workshop. The present work aimed to document the reactivity of the CD80, and CD86 monoclonal antibody (mAb) Panel on T-cell subsets at different times after PHA activation. at different times after PHA activation. at different times after PHA activation. at different times after PHA activation. (PHA) was reported in the Fifth Workshop. The present work aimed to document the reactivity of the CD80, and CD86 monoclonal antibody (mAb) Panel on T-cell subsets at different times after PHA activation. at different times after PHA activation. at different times after PHA activation. at different times after PHA activation. (PHA) was reported in the Fifth Workshop. The present work aimed to document the reactivity of the CD80, and CD86 monoclonal antibody (mAb) Panel on T-cell subsets at different times after PHA activation. at different times after PHA activation. at different times after PHA activation. at different times after PHA activation. (PHA) was reported in the Fifth Workshop. The present work aimed to document the reactivity of the CD80, and CD86 monoclonal antibody (mAb) Panel on T-cell subsets at different times after PHA activation. at different times after PHA activation. at different times after PHA activation. at different times after PHA activation. after three days of activation with phytohemagglutinin (PHA) was reported in the Fifth Workshop. The present work aimed to document the reactivity of the CD80, and CD86 monoclonal antibody (mAb) Panel on T-cell subsets at different times after PHA activation. at different times after PHA activation. at different times after PHA activation. at different times after PHA activation. (PHA) was reported in the Fifth Workshop. The present work aimed to document the reactivity of the CD80, and CD86 monoclonal antibody (mAb) Panel on T-cell subsets at different times after PHA activation. at different times after PHA activation. at different times after PHA activation. at different times after PHA activation. (PHA) was reported in the Fifth Workshop. The present work aimed to document the reactivity of the CD80, and CD86 monoclonal antibody (mAb) Panel on T-cell subsets at different times after PHA activation. at different times after PHA activation. at different times after PHA activation. at different times after PHA activation. (PHA) was reported in the Fifth Workshop. The present work aimed to document the reactivity of the CD80, and CD86 monoclonal antibody (mAb) Panel on T-cell subsets at different times after PHA activation. at different times after PHA activation. at different times after PHA activation. at different times after PHA activation. after three days of activation with phytohemagglutinin (PHA) was reported in the Fifth Workshop. The present work aimed to document the reactivity of the CD80, and CD86 monoclonal antibody (mAb) Panel on T-cell subsets at different times after PHA activation. at different times after PHA activation. at different times after PHA activation. at different times after PHA activation. (PHA) was reported in the Fifth Workshop. The present work aimed to document the reactivity of the CD80, and CD86 monoclonal antibody (mAb) Panel on T-cell subsets at different times after PHA activation. at different times after PHA activation. at different times after PHA activation. at different times after PHA activation. (PHA) was reported in the Fifth Workshop. The present work aimed to document the reactivity of the CD80, and CD86 monoclonal antibody (mAb) Panel on T-cell subsets at different times after PHA activation. at different times after PHA activation. at different times after PHA activation. at different times after PHA activation. (PHA) was reported in the Fifth Workshop. The present work aimed to document the reactivity of the CD80, and CD86 monoclonal antibody (mAb) Panel on T-cell subsets at different times after PHA activation. at different times after PHA activation. at different times after PHA activation. at different times after PHA activation. after three days of activation with phytohemagglutinin (PHA) was reported in the Fifth Workshop. The present work aimed to document the reactivity of the CD80, and CD86 monoclonal antibody (mAb) Panel on T-cell subsets at different times after PHA activation. at different times after PHA activation. at different times after PHA activation. at different times after PHA activation. (PHA) was reported in the Fifth Workshop. The present work aimed to document the reactivity of the CD80, and CD86 monoclonal antibody (mAb) Panel on T-cell subsets at different times after PHA activation. at different times after PHA activation. at different times after PHA activation. at different times after PHA activation. (PHA) was reported in the Fifth Workshop. The present work aimed to document the reactivity of the CD80, and CD86 monoclonal antibody (mAb) Panel on T-cell subsets at different times after PHA activation. at different times after PHA activation. at different times after PHA activation. at different times after PHA activation. (PHA) was reported in the Fifth Workshop. The present work aimed to document the reactivity of the CD80, and CD86 monoclonal antibody (mAb) Panel on T-cell subsets at different times after PHA activation. at different times after PHA activation. at different times after PHA activation. at different times after PHA activation. latter cell type [ll. A modest increase in CD86 expression after three days of activation with phytohemagglutinin (PHA) was reported in the Fifth Workshop. The present work aimed to document the reactivity of the CD80, and CD86 monoclonal antibody (mAb) Panel on T-cell subsets at different times after PHA activation. at different times after PHA activation. at different times after PHA activation. at different times after PHA activation. (PHA) was reported in the Fifth Workshop. The present work aimed to document the reactivity of the CD80, and CD86 monoclonal antibody (mAb) Panel on T-cell subsets at different times after PHA activation. at different times after PHA activation. at different times after PHA activation. at different times after PHA activation. (PHA) was reported in the Fifth Workshop. The present work aimed to document the reactivity of the CD80, and CD86 monoclonal antibody (mAb) Panel on T-cell subsets at different times after PHA activation. at different times after PHA activation. at different times after PHA activation. at different times after PHA activation. (PHA) was reported in the Fifth Workshop. The present work aimed to document the reactivity of the CD80, and CD86 monoclonal antibody (mAb) Panel on T-cell subsets at different times after PHA activation. at different times after PHA activation. at different times after PHA activation. at different times after PHA activation. after three days of activation with phytohemagglutinin (PHA) was reported in the Fifth Workshop. The present work aimed to document the reactivity of the CD80, and CD86 monoclonal antibody (mAb) Panel on T-cell subsets at different times after PHA activation. at different times after PHA activation. at different times after PHA activation. at different times after PHA activation. (PHA) was reported in the Fifth Workshop. The present work aimed to document the reactivity of the CD80, and CD86 monoclonal antibody (mAb) Panel on T-cell subsets at different times after PHA activation. at different times after PHA activation. at different times after PHA activation. at different times after PHA activation. (PHA) was reported in the Fifth Workshop. The present work aimed to document the reactivity of the CD80, and CD86 monoclonal antibody (mAb) Panel on T-cell subsets at different times after PHA activation. at different times after PHA activation. at different times after PHA activation. at different times after PHA activation. (PHA) was reported in the Fifth Workshop. The present work aimed to document the reactivity of the CD80, and CD86 monoclonal antibody (mAb) Panel on T-cell subsets at different times after PHA activation. at different times after PHA activation. at different times after PHA activation. at different times after PHA activation. after three days of activation with phytohemagglutinin (PHA) was reported in the Fifth Workshop. The present work aimed to document the reactivity of the CD80, and CD86 monoclonal antibody (mAb) Panel on T-cell subsets at different times after PHA activation. at different times after PHA activation. at different times after PHA activation. at different times after PHA activation. (PHA) was reported in the Fifth Workshop. The present work aimed to document the reactivity of the CD80, and CD86 monoclonal antibody (mAb) Panel on T-cell subsets at different times after PHA activation. at different times after PHA activation. at different times after PHA activation. at different times after PHA activation. (PHA) was reported in the Fifth Workshop. The present work aimed to document the reactivity of the CD80, and CD86 monoclonal antibody (mAb) Panel on T-cell subsets at different times after PHA activation. at different times after PHA activation. at different times after PHA activation. at different times after PHA activation. (PHA) was reported in the Fifth Workshop. The present work aimed to document the reactivity of the CD80, and CD86 monoclonal antibody (mAb) Panel on T-cell subsets at different times after PHA activation. at different times after PHA activation. at different times after PHA activation. at different times after PHA activation. after three days of activation with phytohemagglutinin (PHA) was reported in the Fifth Workshop. The present work aimed to document the reactivity of the CD80, and CD86 monoclonal antibody (mAb) Panel on T-cell subsets at different times after PHA activation. at different times after PHA activation. at different times after PHA activation. at different times after PHA activation. (PHA) was reported in the Fifth Workshop. The present work aimed to document the reactivity of the CD80, and CD86 monoclonal antibody (mAb) Panel on T-cell subsets at different times after PHA activation. at different times after PHA activation. at different times after PHA activation. at different times after PHA activation. (PHA) was reported in the Fifth Workshop. The present work aimed to document the reactivity of the CD80, and CD86 monoclonal antibody (mAb) Panel on T-cell subsets at different times after PHA activation. at different times after PHA activation. at different times after PHA activation. at different times after PHA activation. (PHA) was reported in the Fifth Workshop. The present work aimed to document the reactivity of the CD80, and CD86 monoclonal antibody (mAb) Panel on T-cell subsets at different times after PHA activation. at different times after PHA activation. at different times after PHA activation. at different times after PHA activation. studies have shown it also to be expressed on mature dendritic cells and activated T cells, although conflicting findings have been reported on the expression of CD86 by the latter cell type [ll. A modest increase in CD86 expression after three days of activation with phytohemagglutinin (PHA) was reported in the Fifth Workshop. The present work aimed to document the reactivity of the CD80, and CD86 monoclonal antibody (mAb) Panel on T-cell subsets at different times after PHA activation. at different times after PHA activation. at different times after PHA activation. at different times after PHA activation. (PHA) was reported in the Fifth Workshop. The present work aimed to document the reactivity of the CD80, and CD86 monoclonal antibody (mAb) Panel on T-cell subsets at different times after PHA activation. at different times after PHA activation. at different times after PHA activation. at different times after PHA activation. (PHA) was reported in the Fifth Workshop. The present work aimed to document the reactivity of the CD80, and CD86 monoclonal antibody (mAb) Panel on T-cell subsets at different times after PHA activation. at different times after PHA activation. at different times after PHA activation. at different times after PHA activation. (PHA) was reported in the Fifth Workshop. The present work aimed to document the reactivity of the CD80, and CD86 monoclonal antibody (mAb) Panel on T-cell subsets at different times after PHA activation. at different times after PHA activation. at different times after PHA activation. at different times after PHA activation. after three days of activation with phytohemagglutinin (PHA) was reported in the Fifth Workshop. The present work aimed to document the reactivity of the CD80, and CD86 monoclonal antibody (mAb) Panel on T-cell subsets at different times after PHA activation. at different times after PHA activation. at different times after PHA activation. at different times after PHA activation. (PHA) was reported in the Fifth Workshop. The present work aimed to document the reactivity of the CD80, and CD86 monoclonal antibody (mAb) Panel on T-cell subsets at different times after PHA activation. at different times after PHA activation. at different times after PHA activation. at different times after PHA activation. (PHA) was reported in the Fifth Workshop. The present work aimed to document the reactivity of the CD80, and CD86 monoclonal antibody (mAb) Panel on T-cell subsets at different times after PHA activation. at different times after PHA activation. at different times after PHA activation. at different times after PHA activation. (PHA) was reported in the Fifth Workshop. The present work aimed to document the reactivity of the CD80, and CD86 monoclonal antibody (mAb) Panel on T-cell subsets at different times after PHA activation. at different times after PHA activation. at different times after PHA activation. at different times after PHA activation. after three days of activation with phytohemagglutinin (PHA) was reported in the Fifth Workshop. The present work aimed to document the reactivity of the CD80, and CD86 monoclonal antibody (mAb) Panel on T-cell subsets at different times after PHA activation. at different times after PHA activation. at different times after PHA activation. at different times after PHA activation. (PHA) was reported in the Fifth Workshop. The present work aimed to document the reactivity of the CD80, and CD86 monoclonal antibody (mAb) Panel on T-cell subsets at different times after PHA activation. at different times after PHA activation. at different times after PHA activation. at different times after PHA activation. (PHA) was reported in the Fifth Workshop. The present work aimed to document the reactivity of the CD80, and CD86 monoclonal antibody (mAb) Panel on T-cell subsets at different times after PHA activation. at different times after PHA activation. at different times after PHA activation. at different times after PHA activation. (PHA) was reported in the Fifth Workshop. The present work aimed to document the reactivity of the CD80, and CD86 monoclonal antibody (mAb) Panel on T-cell subsets at different times after PHA activation. at different times after PHA activation. at different times after PHA activation. at different times after PHA activation. after three days of activation with phytohemagglutinin (PHA) was reported in the Fifth Workshop. The present work aimed to document the reactivity of the CD80, and CD86 monoclonal antibody (mAb) Panel on T-cell subsets at different times after PHA activation. at different times after PHA activation. at different times after PHA activation. at different times after PHA activation. (PHA) was reported in the Fifth Workshop. The present work aimed to document the reactivity of the CD80, and CD86 monoclonal antibody (mAb) Panel on T-cell subsets at different times after PHA activation. at different times after PHA activation. at different times after PHA activation. at different times after PHA activation. (PHA) was reported in the Fifth Workshop. The present work aimed to document the reactivity of the CD80, and CD86 monoclonal antibody (mAb) Panel on T-cell subsets at different times after PHA activation. at different times after PHA activation. at different times after PHA activation. at different times after PHA activation. (PHA) was reported in the Fifth Workshop. The present work aimed to document the reactivity of the CD80, and CD86 monoclonal antibody (mAb) Panel on T-cell subsets at different times after PHA activation. at different times after PHA activation. at different times after PHA activation. at different times after PHA activation. latter cell type [ll. A modest increase in CD86 expression after three days of activation with phytohemagglutinin (PHA) was reported in the Fifth Workshop. The present work aimed to document the reac