INVESTIGADORES
SALAMONE Gabriela Veronica
artículos
Título:
Thioperamide induces CD4 CD25 Foxp3 regulatory T lymphocytes in the lung mucosa of allergic mice through its action on dendritic cells
Autor/es:
AMARAL MM, ALVAREZ CA, LANGELLOTTI C, JANCIC C, SALAMONE G, GEFFNER J, VERMEULEN M.
Revista:
J Asthma Allergy
Editorial:
Dove Medical Press
Referencias:
Año: 2011 vol. 4 p. 93 - 102
ISSN:
1178-6965
Resumen:
Background: Histamine is an important mediator in the development of allergic reactions. The
biological effects of histamine are mediated through four histaminergic receptors. In recent years,
an important role has been assigned to the proinflammatory functions of histamine regarding the
H4 receptor. Previously, we have demonstrated that injection of immature dendritic cells treated
with histamine into allergic mice promotes an increase in CD8+ Tc2 lymphocytes, which are
involved in the worsening of allergy symptoms during the chronic phase of the disease. The aim of
this study was to evaluate the role of the H3/H4 receptor antagonist, thioperamide, in allergy.
Methods: Ovalbumin-allergized mice and nonallergized mice were injected with phosphatebuffered
saline, dendritic cells, or thioperamide-treated dendritic cells. After treatment, the lungs
of the mice were obtained and analyzed for changes in the populations of dendritic cells and T
lymphocytes, as well as the expression of H and H4 receptors in mononuclear lung cells.
Results: We found an increase in regulatory T cells in the lungs of allergic mice intratracheally
injected with dendritic cells which had their H3/H4 receptors blocked with thioperamide. We
also found an increase in the production of interleukin-10 by dendritic cells of the lung. Finally,
we observed a decrease in serum levels of specific anti-IgE and a reduction of eosinophils in
bronchoalveolar lavage from allergic mice.
Conclusion: Thioperamide induces a significant improvement in symptoms of allergic reaction
perhaps via induction of regulatory T lymphocytes. These findings may become relevant in the
understanding of type 1 hypersensivity reactions.