Proteome analysis of human bronchial epithelial cells infected with Bordetella pertussis.

MARTINA DEBANDI; MANUELA GESELL SALAZAR; CARLOS BAROLI; CHRISTIAN HENTSCHKER; MARIELA CARRICA; FRANK SCHMIDT; MARIA EUGENIA RODRIGUEZ; UWE VÖLKER; SURMANN KRISTIN; YANINA LAMBERTI

Mar del Plata

Congreso; LXIV Reunión Anual de la Sociedad Argentina de Inmunología (SAI). Reunion Conjunta SAI SAIC SAFIS; 2018

Sociedad Argentina de Inmunología

Bordetella pertussis (Bp), the causative agent of whooping cough, survives within host cells potentially contributing to its ability to persist within hosts and populations. Pertussis toxin (PT) plays a major role in the modulation of macrophage responses and intracelular survival. The airway epithelium is also considered central to the orchestration of the immune responses, yet their responses to Bp have not been fully investigated. In this study, we examined the molecular responses of the human bronchial epithelial cell line 16HBE14o- to Bp infection by shotgun proteomics. To this end, 16HBE14o- cells were infected with Bp wild type (wt) or an isogenic mutant lacking PT (ΔPT) during 4.5 h and 8 h at a multiplicity of infection of one and the host response was compared to a similarly treated uninfected control. Among 2425 identified proteins, the abundance levels of 745 proteins were significantly altered by Bp infection compared with the control (p