SUPRAMOLECULAR ARCHITECTURE BASED ON THE SELF-ASSEMBLING OF MULTI-WALL CARBON NANOTUBES DISPERSED IN POLYHISTIDINE AND GLUCOSE OXIDASE: CHARACTERIZATION AND ANALYTICAL APPLICATIONS FOR GLUCOSE BIOSENSING

DALMASSO PABLO; M. L. PEDANO; G. A. RIVAS

BIOSENSORS & BIOELECTRONICS

ELSEVIER ADVANCED TECHNOLOGY

Lugar: Amsterdam; Año: 2012

0956-5663

We reportforthefirsttimethedevelopmentofasensitiveandselectiveglucosebiosensorbasedonthe self-assemblingofmultiwallcarbonnanotubes(MWCNTs)dispersedinpolyhistidine(Polyhis)and glucoseoxidase(GOx)onglassycarbonelectrodes(GCE).Thesupramoleculararchitecturewas characterizedbySEM,FT-IRandelectrochemicaltechniques.Theoptimummultistructurewas obtainedwithfive(MWCNT-Polyhis/GOx)bilayersandonelayerofNafionasanti-interferentbarrier. The sensitivityat0.700Vwas(1.9470.03) mAM1 (r¼0.9991),withalinearrangebetween0.25and 5.00 mM,adetectionlimitof2.2 mM andaquantificationlimitof6.7 mM withminimuminterference from lactose(1.5%),maltose(5.7%),galactose(1.2%),ascorbicacid(1.0%),anduricacid(3.3%).The biocatalyticlayerdemonstratedtobehighlyreproduciblesincetheR.S.D.for10successiveampero- metriccalibrationsusingthesamesurfacewas3.6%.Thesensitivityofthebiosensorafter15day storageat4 1C remainedat90%ofitsoriginalvalue.Thecombinationoftheexcellentdispersing propertiesandpolycationicnatureofpolyhistidine,thestabilityoftheMWCNT-Polyhisdispersion,the electrocatalyticpropertiesofMWCNTs,thebiocatalyticspecificityofGOx,andthepermselective propertiesofNafionhaveallowedbuildingupasensitive,selective,robust,reproducibleandstable glucoseamperometricbiosensorforthequantificationofglucoseinmilksamples. 70.03) mAM1 (r¼0.9991),withalinearrangebetween0.25and 5.00 mM,adetectionlimitof2.2 mM andaquantificationlimitof6.7 mM withminimuminterference from lactose(1.5%),maltose(5.7%),galactose(1.2%),ascorbicacid(1.0%),anduricacid(3.3%).The biocatalyticlayerdemonstratedtobehighlyreproduciblesincetheR.S.D.for10successiveampero- metriccalibrationsusingthesamesurfacewas3.6%.Thesensitivityofthebiosensorafter15day storageat4 1C remainedat90%ofitsoriginalvalue.Thecombinationoftheexcellentdispersing propertiesandpolycationicnatureofpolyhistidine,thestabilityoftheMWCNT-Polyhisdispersion,the electrocatalyticpropertiesofMWCNTs,thebiocatalyticspecificityofGOx,andthepermselective propertiesofNafionhaveallowedbuildingupasensitive,selective,robust,reproducibleandstable glucoseamperometricbiosensorforthequantificationofglucoseinmilksamples.