INVESTIGADORES
PUNTARULO Susana Angela
artículos
Título:
OXIDATIVE STRESS ASSESSED IN SALIVA FROM PATIENTS WITH
Autor/es:
MARIA C RUBIO; PAULA M GONZALEZ; CECILLIA RAMOS; PABLO LEWIN; SILVIA FRIEDMAN; SUSANA PUNTARULO; LILIANA NICOLOSI
Revista:
ACTA ODONTOLOGICA LATINOAMERICANA : AOL.
Editorial:
Sociedad Argentina de Investigacion Odontologica
Referencias:
Lugar: Buenos Aires; Año: 2013 vol. 26 p. 116 - 120
ISSN:
0326-4815
Resumen:
There is evidence that acute myocardial infarction (AMI) is associated
with increasing production of reactive oxygen species and
tissue injury. The aim of this study was to assess the presence of
oxidative stress indices in saliva 24 and 48h after AMI. Materials
and methods: We designed a prospective study comparing salivary
levels of biomarkers of oxidative stress in patients with AMI
with elevation of the ST segment in electrocardiogram versus clinically
healthy subjects. Oxidative stress indices including the rate
of oxidation of 2?7? dichlorohydrofluorescein diacetate (DCFHDA)
and the activity of the antioxidant enzyme catalase (CAT)
were evaluated in saliva from patients with AMI at 24 and 48
hours. At each sampling time, blood was drawn for serum markers
of myocardial infarction. Results: This study included ten
patients with acute ST-segment elevation myocardial infarction
and ten clinically healthy controls. Mean age was 67.8 ± 11.1 vs.
48.7 ± 4.1 years (p<0.001) and gender was 60% male vs. 50%
(p>0.05) for AMI vs. controls, respectively. Our results demonstrated
an increase in the rate of oxidation of DCFH-DA in the
myocardial infarction group as compared with controls (p=0.004),
which remained unchanged at 48h. There was no difference in
salivary catalase activity between controls and AMI subjects at
24h or at 48h post-diagnosis (p=0.157). The relationship between
CAT48 and DCFH-DA48 was fairly significant (r=0.39; p=0.053).
Conclusion: This preliminary study showed that biomarkers of
oxidative stress are detectable in saliva of patients with acute
myocardial infarction. Clinical Relevance: Future studies using a
larger population are needed to confirm these observations and to
explore the possibility of using the saliva to monitor evolving diagnosis
and prognosis in acute coronary syndrome.