Chloroplast as a Nitric Oxide Cellular Source. Effect of Reactive Nitrogen Species on Chlroplastic Lipids and Proteins

JASID S. ; SIMONTACCHI M.; BARTOLI C.G. ; PUNTARULO S.

PLANT PHYSIOLOGY.

AMER SOC PLANT BIOLOGISTS

Año: 2006 vol. 142 p. 1246 - 1255

0032-0889

Puntarulo S.NO) generation by soybean (Glycine max, var ADM 4800) chloroplasts was studied as an endogenous product assessed by electron paramagnetic resonance (EPR) spin-trapping technique. Nitrite or L-arginine (Arg) are the substrates for enzymatic activities considered to be the possible sources of NO in plants. Soybean chloroplasts showed a NO production of 3.2 ± 0.2 nmol min-1 mg-1 protein in the presence of 1 mM NaNO2. Inhibition of photosynthetic electron flow by DCMU resulted in a lower rate (1.21 ± 0.04 nmol min-1 mg-1 prot) of NO generation. Chloroplasts incubated with 1 mM Arg showed a NO production of 0.76 ± 0.04 nmol min-1 mg-1 protein. This production was inhibited when chloroplasts were incubated in presence of NOS-inhibitors L-NAME and L-NNA. In vitro exposure of chloroplasts to an NO-donor (250 m M GSNO) decreased lipid radical content in membranes by 29%, however, incubation in the presence of 25 m M peroxynitrite (ONOO-) led to an increase in lipid-derived radicals (34%). The effect of ONOO- on protein oxidation was determined by western blotting, showing an increase in carbonyl content either in stroma or thylakoid proteins as compared to control. Moreover, ONOO- treatment significantly affected both O2 evolution and chlorophyll fluorescence in thylakoids. Data reported here suggest that NO is an endogenous metabolite in soybean chloroplasts and that reactive nitrogen species could exert either antioxidant or prooxidant effects on chloroplast macromolecules.