Color changes and shear bond strength to simulated caries lesions treated with a novel solution of 20% silver nanoclusters in polymethacrylic acid

G. F. MOLINA; M. B. CABALÉN; J. P. ARANGUREN; S. D. PALMA; G. A. PINO; M. F. BURROW

Scientific Reports

Nature Publishing Group

Año: 2022 vol. 12

2045-2322

Objectives: (1) To compare the staining effect on demineralized dentin simulating caries between silver nanoclusters (AgNCls) synthesized using polymethacrylic acid (PMAA) and silver diammine fluoride (SDF), and (2) to measure the shear bond strength (SBS) of a glass ionomer cement (GIC) to simulated caries lesions with and without the application of AgNCls/PMAA or SDF. Methods: Dentine blocks 4 mm thick from twenty-four non-carious third molars were sectioned and coated with nail varnish (Revlon, New York, USA). Simulated caries lesions on occlusal dentin surfaces were created (66 hours in 0.05M acetate buffer 2.2 mM calcium/phosphate pH 5.0). Specimens were divided into groups and treated with (n=8): A) 20% AgNCls/PMAA; B) SDF 38% (Fagamin, Tedequim, Córdoba, Argentina); or C) without treatment. AgNCls/PMAA or SDF were applied on the exposed surfaces with a microbrush for 10s. Color of the surface was measured according to the CIE-L*a*b* system before and after demineralization (R0 and R1), immediately and one week after treatment (R2 and R3), using a spectrophotometer (CM-600D Konica Minolta Sesing Inc., Japan). Groups A and B received an extra application of AgNCls/PMAA or SDF before a conventional GIC (Fuji IX-Gold Label, GC Corp, Tokyo, Japan) was bonded using a mould, 4mm diameter × 3mm high. Samples were incubated for 24 hours at 37°C in 100% relative humidity. For SBS, a Universal Testing Machine (Digimess RS-8000-5, China) - crosshead speed of 1mm/min- was used. Statistical analysis was performed using ANOVA, Student-t and Scheffe-test at a significance of p