Antifungal activity of silver nanoparticles and clotrimazole against Candida spp.

MARÍA LAURA MENESES; MAITE RECALDE; PAULA LORENA MARTIN; ALEJANDRO GUILLERMO PARDO

BRAZILIAN JOURNAL OF PHARMACEUTICAL SCIENCES

UNIV SAO PAULO

Lugar: Ribeirao Preto; Año: 2020

1984-8250

The aim of present study was calculate the Minimum inhibitory concentrations (MICs)of silver nanoparticles and clotrimazole for Candida species and their interaction by theadaptation of standarized methods.The MICs values of clotrimazole were 9 E-04-3 E-03 ug/ml, 0.1-0.6 ug/ml, 3 E-03- 0.1 ug/ml and 3 E-03-0.3 ug/ml for Candida albicans susceptible to fluconazole, Candidaalbicans resistance to fluconazole, Candida krusei and Candida parapsilosisrespectively. The MICs values of silver nanoparticles were 26.50- 53 ug/ml; 26.50-106ug/ml; 106-212 ug/ml and 26.50- 53 ug/ml for Candida albicans susceptible tofluconazole, Candida albicans resistance to fluconazole, Candida krusei and Candidaparapsilosis respectively. Synergism between clotrimazole and silver nanoparticles wasmeasured by checkerboard BMD (broth microdilution) test and shown only for C.albicans susceptible to fluconazole because the fractional inhibitory concentrations(FICs) values were 0.07 - 0.15 ug/ml. Indifference was shown for the other speciestested because the FICs values were between 0.5 ? 2- 3.06 ug/ml.The results suggest synergistic activity depending on the fungus species analysed,however we recommend the incorporation of others measurement methodologies toconfirm our results.As for measurement methodologies of MICs of silver nanoparticles and clotrimazoleinternational normative were respected to guarantee reproducible and comparableresults.