Intra- and Extracellular Biosynthesis and Characterization of Iron Nanoparticles from Prokaryotic Microorganisms with Anticoagulant Activity

KARINA CRESPO, JOSÉ BARONETTI, MELISA QUINTEROS, PAULINA PÁEZ, MARÍA GABRIELA PARAJE

PHARMACEUTICAL RESEARCH

SPRINGER/PLENUM PUBLISHERS

Lugar: New York; Año: 2017

0724-8741

BackgroundThe use ofmicroorganisms for the synthesis of nanoparticles (NPs) is relatively new inbasic research and technology areas.PurposeThis workwas conducted to optimized the biosynthesis of iron NPs intra- andextracellular by Escherichia coli or Pseudomonas aeruginosa and to evaluatetheir anticoagulant activity.StudyDesign/MethodsThestructures and properties of the iron NPs were investigated byUltraviolet?visible (UV-vis) spectroscopy, Zeta potential, Dynamic lightscattering (DLS), Field emission scanning electron microscope (FESEM)/ Energydispersive X-ray (EDX) and transmission electron microscopy (TEM).Anticoagulant activity was determined by conducting trials of Thrombin Time(TT), Activated Partial Prothrombin Time (APTT) and Prothrombin Time (PT).ResultsUV-vis spectrumof the aqueous medium containing iron NPs showed a peak at 275 nm. The formingof iron NPs was confirmed by FESEM/ EDX, and TEM. The morphology was sphericalshapes mostly with low polydispersity and the average particle diameter was23 ± 1 nm. Iron NPs showed anticoagulant activity by the activation ofextrinsic pathway.ConclusionTheeco-friendly process of biosynthesis of iron NPs employing prokaryoticmicroorganisms presents a good anticoagulant activity. This could be exploredas promising candidates for a variety of biomedical and pharmaceuticalapplications.KEY WORDSanticoagulantactivityEscherichiacoliiron nanoparticlesmicrobial biosynthesisPseudomonas aeruginosa