Fungicidal and anti-biofilm activities of gold nanoparticles on Candida tropicalis

MARÍA ANGEL DA SILVA, JOAQUÍN CALIZTO GARCÍA-MARTÍNEZ, PAULINA LAURA PÁEZAND MARÍA GABRIELA PARAJE

NANOMEDICINE

FUTURE MEDICINE LTD

Lugar: Londres; Año: 2022 vol. 17 p. 1663 - 1676

1743-5889

Aim: To investigate the antifungal activity of two different functionalized gold nanoparticles (AuNPs), stabilized with cetyltrimethylammonium bromide (CTAB-AuNPs) and conjugated with cysteine (cys-AuNPs), and their effects on the architecture of Candida tropicalis biofilms. Materials & methods: Biofilms were studied by Crystal violet binding assay and scanning electron microscopy (SEM). We investigated the effects of AuNPs on reactive oxygen species (ROS), reactive nitrogen intermediates (RNI), and enzymatic and non-enzymatic antioxidant defenses. Results/Conclusions: The fungicidal activity and cellular stress of both AuNPs affected biofilm growth through accumulation of ROS and RNI. However, CTAB-AuNPs revealed a higher redox imbalance. We correlated, for the first time, AuNP effects with the redox imbalance and alterations in the architecture of C. tropicalis biofilms.