INVESTIGADORES
PANZETTA-DUTARI Graciela Maria Del Valle
artículos
Título:
Restriction fragment Length Polymorphism of the mtDNA A+T rich region as a genetic marker in Aedes aegypti.
Autor/es:
RONDAN DUEÑAS, J ; PANZETTA DE DUTARI, GM; BLANCO, A; GARDENAL, CN
Revista:
ANNALS OF THE ENTOMOLOGYCAL SOCIETY OF AMERICA
Editorial:
ENTOMOLOGICAL SOC AMER
Referencias:
Año: 2002 vol. 95 p. 352 - 358
ISSN:
0013-8746
Resumen:
ABSTRACT The usefulness of the control region of mtDNA as a tool for the study of population
structure in the mosquito Aedes aegypti (L.) was analyzed. Population samples were taken from several
geographic areas of Argentina; one additional sample from the city of San Juan, Puerto Rico, was
included for comparison. In individual mosquitoes, the AT-rich and the COII-COIII regions were
ampliÞed by polymerase chain reaction using universal insect primers. Restriction fragment-length
polymorphisms were investigated from ampliÞcation products. In the AT region, three ampliÞed
fragments of different total length were obtained (of 2,500, 2,300, and 2,100 bp). The enzymes Ssp
I, Dra I, Pac I, and Apo I produced polymorphic patterns. Including total fragment length as a variable,
eight different haplotypes were resolved for Argentinian populations, some presenting a restricted
geographic distribution. The coding COII-COIII region revealed very low polymorphism. Although
11 restriction enzymes were employed to analyze this region, only two different haplotypes were
found, one of them shared by populations as distant as Buenos Aires and San Juan. We demonstrated
that total length and restriction fragment-length polymorphisms within the noncoding AT-rich
region, but not the coding COII-COIII fragment, are informative to discriminate Aedes aegypti
populations. Mean FST value (0.48, P0.001) indicates an important degree of differentiation among
populations. Absence of an isolation by distance pattern was demonstrated.