Effects of body condition score in liver proinflammatory state and insulin-signaling of dairy cattle during the transition period.

ANGELI E; BARCAROLO, D; NOTARO, U; MATILLER, V; REY, F; ORTEGA, HUGO H.; HEIN, G.J.

Mar del Plata

Congreso; LXIII Reunión Científica Anual de la Sociedad Argentina de Investigación Clínica (SAIC), LXVI Reunión anual de la SAI; Reunión anual de la SAFIS; con la participación de la SAV, AACYTAL y Asociacion Argentina de Nanomedicinas (NANOMEDAR).; 2019

Sociedad Argentina de Investigación Clínica (SAIC)

The transition period (TP) is the most critical stage in the lactation of adairy cow, characterized by lipid mobilization, proinflammatory state and insulinresistance during peripartum [1]. The aim of this study was to evaluatethe insulin-signaling pathway and certain pro-inflammatory constituents in the  liver and plasma parameters during the TPin cows with different body condition score (BCS). Sixteen grazing dairy cowsfrom a commercial dairy farm were classified according to BCS using the 5-pointscale [2] as high BCS (HBCS, ≥ 3.5, n = 8,) and lowBCS (LBCS, < 3.5, n = 8). Blood and liver biopsies were sampled at -14, 4,14 and 28 days relative to parturition. The concentrations of NEFA, BHB, glucose, triglycerides, insulin and liver triglycerideswere spectrophotometrically measured. Also, liver protein expression of relevantinsulin signaling components: insulin receptor (IR), insulin receptor substrate1 (IRS-1), total and phosphorylated protein kinase B (Akt and p-Akt); alongwith tumor necrosisfactor alpha (TNFα), interleukin-6 (IL-6), and nuclear factor-κB (NF-κB)were measured by Western blot. Results obtained shown that cows with HBCShad greater BHB concentration than LBCS (p < 0.05); an interaction effect (BCSx time, p < 0.05) for NEFA and TNFα was determined; protein abundance of thisparameter  was greater at day 4 postpartum inHBCS group (p < 0.05). In addition, cows with HBCS had a tendency to have a greaterp-Akt/Akt than those with LBCS (p = 0.08). These results suggest that the proinflammatorystate in liver of dairy cows with HBCS during postpatum could affect the insulinsignaling in agreement with reports of other researchers [3]. This knowledge could help to understandthe metabolic behavior and to optimize the health and milk production of dairycattle during this periodo