Reactivity of phagocytic cells during lactation of dairy cows: their implication on the establishment of pregnancy

PERALTA MB; CAINELLI S; ANGELI E; STASSI AF; SALVETTI NR; ORTEGA HH; REY F; VELAZQUEZ M

Tucuman

Congreso; LXVII Reunión Anual de la Sociedad Argentina de Inmunología (SAI); 2019

Sociedad Argentina de Inmunología (SAI)

index of days to conception considering the hormonal and metabolic context. Bloodsamples were collected at different postpartum days. Cows were grouped according to thelength of days to conception period: fewer (83±6 days) and greater (128±32) days. Allprocedures were approved by the Ethics Committee (FCV-UNL). Plasmatic concentrations of cortisol, estrogen, progesterone, non-esterified fatty acids and glucose were measured. The differential leukocyte counts were performed by blood smears. The PA and mean fluorescence intensity (MFI) of monocytes/macrophages were determined by flow cytometry. A Generalized Linear Model with repeated measures was used to analyze different effect on leukocyte count and immunoreactivity. Kaplan?Meier test was used to evaluate the effect of PA and MFI on the delay to conception time. The results showed that the hormonal and metabolites levels there were no significant effects on the counts of monocytes/macrophages over time. However, estradiol and glucose concentrations had a significant effect on the PA and MFI. Finally, cows with lower MFI were pregnant earlier than those with higher MFI. These results suggest a relation between the reactivity of monocytes/macrophages and the delay to conception time.