Altered expression of steroid hormones receptors coregulators during bovine cystic ovarian disease development

NOTARO, U; HUBER E; SALVETTI NR; ORTEGA, H.H; REY F; RODRIGUEZ, F.M.; RECCE, S; BARBERIS F

Mar del Plata

Congreso; LXI Reunión Científica Anual de la Sociedad Argentina de Investigación Clínica (SAIC), LXIV Reunión anual de la SAI; XLVIII Reunión anual de la SAFE; VII Reunión anual de la NANOMEDAR; 2016

Steroid hormones regulate importantreproduction events through its nuclear receptors. Steroid hormones receptorsare ligand regulated transcription factors which modulate genic transcriptionthrough molecular mechanisms associated with coregulatory proteins(coactivators and corepresors). The endocrine profile, growth dynamics, andhistologic characteristics of persistent ovarian follicles are analogous tothose of spontaneous cysts. Cystic Ovarian Disease (COD) is a major factorcontributing to poor reproductive efficiency of lactating dairy cow. Ourpurpose was to study the protein expression of Steroid Receptor Coactivator-1(SRC-1) and corepressors Receptor-interacting Protein 140 (RIP140) and Liganddependent nuclear receptor Corepressor (LCOR) in the ovaries of healthy cows(Control group) and in an experimental model of follicular persistence inducedby low levels of progesterone. This was achieved by indirect immunohistochemistryand digital image analysis of granulosa cells (GC) and theca cells (TC) in differentfollicular categories:  antral folliclesof the control group as reference structure (AC), persistent follicles ofgroups with 0 (P0, expected day of ovulation), 5 (P5), 10 (P10) and 15 (P15)days of persistence. Expression of SRC-1 was lower in AC than in P0 and P15, inboth GC and TC (p<0.05). Expression of LCOR was higher in GC of AC and P0than P10 (p<0.05), with no differences in TC (p>0.05). Expression ofRIP140 was similar in all analyzed follicular categories (p>0.05), in bothGC and TC. These results suggest that changes in the expression of coregulatoryproteins can lead to altered response to steroid hormones, and thus contributeto the pathogenesis of ovarian alterations such as follicular persistence and COD.