Modified expression of BMP receptor 1B (BMPR1B) during bovine cystic ovarian disease development

DIAZ, PU; GAREIS N; LEIVA, C; REY, F; BERTOLI, J; CATTANEO, L; SALVETTI NR; ORTEGA, H.H

Mar del Plata

Congreso; LXI Reunión Científica Anual de la Sociedad Argentina de Investigación Clínica (SAIC), LXIV Reunión anual de la SAI; XLVIII Reunión anual de la SAFE; VII Reunión anual de la NANOMEDAR; 2016

Sociedad Argentina de Investigación Clínica (SAIC).

Follicular persistence and C􀀱􀀦 are causes of reproductivealterations in dairy cattle. 􀀫n the ovary the bone morphogeneticproteins 􀀊􀀤􀀯􀀲s􀀋 are expressed in granulosa cells 􀀊􀀩C􀀋 andtheca cells 􀀊TC􀀋, and have important actions during folliculardevelopment through binding to a high affinity heterotetramericcomplex of type 􀀫 and type 􀀫􀀫 receptors. 􀀱ur objective 􀁙as tostudy the immunolocalization of 􀀤􀀯􀀲 receptor 􀀓􀀤 􀀊􀀤􀀯􀀲􀀴􀀓􀀤􀀋 inthe ovaries of healthy co􀁙s and animals 􀁙ith spontaneous orACTH induced C􀀱􀀦. 􀀫n addition, 􀁙e evaluated the expressionof 􀀤􀀯􀀲􀀴􀀓􀀤 in an experimental model of follicular persistence inducedby lo􀁙 levels of progesterone. 􀀫n all groups 􀁙e determine dthe expression of 􀀤􀀯􀀲􀀴􀀓􀀤 by indirect immunohistochemistryand digital image analysis in different follicular categories. 􀀹eobserved differences in the C􀀩 of small preantral follicles, 􀁙ithhigher expression in the ACTH􀀏induced and spontaneous C􀀱􀀦groups than in the control group 􀀊p􀀞􀀒.􀀒􀀗􀀋. Comparison of cysts􀁙ith reference structures 􀀊small and large antral follicles􀀋 fromthe control group sho􀁙ed that ACTH􀀏induced and spontaneouscysts presented higher 􀀤􀀯􀀲􀀴􀀓􀀤 expression than small antralfollicles from the control group 􀀊p􀀞􀀒.􀀒􀀗􀀋. 􀀫n theca cells, 􀀤􀀯􀀲􀀴􀀓􀀤expression 􀁙as higher in atretic follicles from the ACTH􀀏induce dgroup than in those from the spontaneous C􀀱􀀦 group 􀀊p􀀞􀀒.􀀒􀀗􀀋,􀁙ithout differences 􀁙ith the control group 􀀊p􀀞􀀒.􀀒􀀗􀀋. Comparisonbet􀁙een control and progesterone􀀏induced persistence groupsfor each follicular category sho􀁙ed higher 􀀤􀀯􀀲􀀴􀀓􀀤 expression ingranulosa cells of atretic follicles of groups 􀁙ith 􀀗 and 􀀓􀀗 da ys offollicular persistence than in those of the control group 􀀊p􀀞􀀒. 􀀒􀀗􀀋.These results suggest that changes in the expression of 􀀤􀀯􀀲􀀴􀀓􀀤can lead to an alteration in ovarian response to BMPs and thuscontribute to the pathogenesis of ovarian alterations as follicularpersistence and C􀀱􀀦.65 9