METFORMIN TREATMENT MODULATES MACROPHAGE RESPONSE AGAINST T. CRUZI INFECTION

BAIGORRI, E; ANA, Y; BRUGO, MB; VIANO, ESTEFANIA; RODRIGUEZ-GALAN, MARIA CECILIA; CLAUDIA MOTRAN; STEMPIN, CINTHIA C.; CERBAN, FABIO MARCELO

Virtual

Congreso; Reunión anual de Sociedades de Biociencias; 2021

SAI-SAIC

In acute phase of T. cruzi infection, both innate and adaptive immunityare necessary to control parasite replication. Macrophage (Mf)and T cells orchestrate the inflammatory response that controls parasiteburden. However, an exacerbated immune response results intissue damage, mainly by ROS and RNS release. Metformin (Mf), atype 2 diabetes drug, reduces inflammation in models of aging andpollution. In our in vivo model of T. cruzi infection in Balb/c mice,we observed that peritoneal and spleen Mf increase iNOS expressionduring acute phase. Pretreatment of BMDM with Mf preventsintracellular parasite replication and promotes proinflammatory cytokineproduction. We also infected RAW cells and then were treatedwith PBS or Mf 1mM. This treatment decreased ROS production(p