Cathepsin L3 from Fasciola hepatica induces IFN-g response in CD4 and CD8 T cells

CELIAS, D; CORVO, ILEANA; TORT, JOSE; SILVANE, LEONARDO; MOTRAN, CC; CERVI, LAP

CABA

Congreso; I meeting LASID SAI FAIC; 2015

SAI LASID

Cathepsin L3 (CL3) from Fasciola hepatica is highlyexpressed in the juvenile larvae. CL3 is a cystein protease withcollagenolytic activity, however there is no information about itsinteraction with the immune system. The aim of this work was tostudy the ability of CL3 to modulate bone marrow-deriveddendritic cells (DC) maturation and the capacity of these cells tomodify T cell responses. Besides we studied the effect of CL3 onT cell from mice infected with the parasite.DC from mice C57BL/6 mice were differentiated with GM-CSFfactor and cultured for 18 h with CL3 produced in Hansenulapolymorpha. These cells were then cultured with allogeneicsplenocytes. Besides, splenocytes from infected mice were restimulatedor not with CL3. Cytokine production was detected byELISA or FACS analysis.CL3 increases the expression of MHC class II in DC, whereasdecreases the expression of the costimulatory molecule CD80.Moreover, these cells increase IFN-γ production in cultures withCD8 and CD4 allogeneic T cells (Student test, p