Cathepsin L3 from Fasciola hepatica induces IL-6 and IL-10 production in murine dendritic cells through the toll-like receptor 4 signaling

CELIAS, D; CORVO, ILEANA; TORT, JOSE; SILVANE, LEONARDO; MOTRAN, CLAUDIA CRISTINA; CERVI, LAP

Mar del Plata

Congreso; LXII Reunión científica de la Sociedad Argentina de Inmunología y LIX REUNIÓN CIENTÍFICA ANUAL Sociedad Argentina de Investigación Clínica; 2014

SAI-SAIC

During its migration through host tissue, the helminth parasite F. hepatica, excrete-secrete a number of products among which are cathepsins. These enzymes belong to the family of cysteineproteases that are essential for survival and colonization of the parasite. While Cathepsin L1 is mainly secreted by adult parasites, Cathepsin L3 (CL3) is released by juvenile worms. Recently, it has been described a collagenolytic activity of this enzyme, wh ich may be relevant in the parasite?s ability to cross the intestinal wall, thus continuing with its life cycle in the host. However, there is no information about the interaction of this protein with immune cells system. Playing dendritic cells (DC) a key role for sensing antigens and inducing different adaptive responses, the aim of this work was to investigate the ability of CL3 to modulate the murine DC maturation and its interaction with TLR receptors. Bone marrow-derived DC from mice C57BL/6 mice were differentiated with GM-CSF factor and cultured for 18 h with recombinant CL3, produced in Hansenula polymorpha. After this time, CL3 didn?t show cytotoxic activity on DC compared to untreated DC (Student?s test p = NS), by cell viability assay with MTT 3 (4,5-di-methyl-thiazol-2-yl) -2,5-di-phenyl tetrazoliumbromide. We also demonstrated that CL3 increases the levels of IL-6 and IL-10 (Student test, p