KUNITZ TYPE MOLECULE-BASED VACCINE ELICITS ELEVATED SPECIFIC IGGS AND IGA ANTIBODIES AND TH1/TH17 RESPONSES AGAINST FASCIOLA HEPATICA

SILVANE, LEONARDO; CELIAS, D; MALETTO, BELKYS; SANCHEZ VALLECILLOS, FERNANDA; CHIDETTI, ANA; ROMAGNOLI, PABLO; MOTRAN, CLAUDIA C; PALMA, SANTIAGO; ALLEMANDI, DANIEL; CERVI, LAURA

CABA

Congreso; Reunión Conjunta de Sociedades de Biociencias; 2017

SAIC-SAI

Fasciola hepatica is a helminth parasite with a worldwidedistribution, which can cause a chronic liver disease: fasciolosis,leading to economic losses in the livestock and public health inmany countries. The prevailing control strategy based on anthelminticdrugs is unsustainable due to widespread resistance; hence vaccinationappears as an attractive option to pursue. In our laboratorywe study a vaccine formulated with Kunitz type molecule (KTM), aninhibitor of serine proteases with a key role in the parasite survival,and CpG-ODN/Coa-ASC16, an adjuvant with capacity to induce Th1and Th17 responses.Previous results showed the efficacy of the vaccination in micewhich generates a reduction in the liver damage produced by the infection.The aim of this work was to investigate the immune responsedeveloped during KTM/CpG-ODN/COA-ASC16 immunizationAnimals were allocated in four groups of 4 each. BALB/c micewere subcutaneously inoculated with 10 μg of KTM in CpG-ODN/Coa-ASC16 on days 0-7-14. Control animals were inoculated thesame days with CpG-ODN/Coa-ASC16 or PBS. On day 21, all animalswere orally infected with 6 F. hepatica metacercariae. An additionalgroup without treatment or infection was including as control.After 4 days of challenge the mice were euthanized.Mice vaccinated with KTM/CpG-ODN/Coa-ASC16 showed highlevels of specific IgG1, IgG2a and IgA in plasma (Student test