INVESTIGADORES
MIRANDA Leandro Andres
artículos
Título:
Ultrastructure of fresh and post thawed sperm of pejerrey Odontesthes bonariensis (Atheriniformes)
Autor/es:
GARRIZ A; MIRANDA LA
Revista:
NEOTROPICAL ICHTHYOLOGY
Editorial:
SOC BRASILEIRA ICTIOLOGIA
Referencias:
Lugar: Porto Alegre; Año: 2013 vol. 11 p. 831 - 836
ISSN:
1679-6225
Resumen:
In the present study it was showed for the first time the ultrastructural morphology of O.
bonariensis sperm using electron microscopy techniques. Different kinds of
abnormalities were described in fresh and post thawed sperm caused by crogenic
protocols. Pejerrey spermatozoon is uniflagellated and is differentiated into 3
parts: a small roundish head (~1.80µm in length and 1.67µm in width), a
midpiece or transitional region (~1.11µm in length and 1.56µm in width), and a
long tail or flagellum (~29.08µm). Samples of fresh and post thawed sperm showed
evidence of morphological anomalies affecting various intracellular
compartments. Spermatozoa with swollen, ruptured, or absent membranes in the
head showing excess of cytoplasm, and with alteration of the spatial
orientation of the mitochondria were observed. A swollen flagellum was observed
containing cytoplasmic vesicles, distributed along the whole length or
concentrated in a restricted part of the tail. It was also found a high level
of abnormalities (60%) in frozen sperm when compared with normal sperm (18%)
reflecting the damage provoked by cryopreservation procedures.