Sensitivity of Bidens laevis L. to mutagenic compounds. Use of chromosomal

PÉREZ, DÉBORA JESABEL; LUKASZEWICZ, GERMÁN; MENONE, MIRTA LUJÁN; CAMADRO, ELSA LUCILA

ENVIRONMENTAL POLLUTION

ELSEVIER SCI LTD

Año: 2011 vol. 159 p. 281 - 286

0269-7491

The wetland macrophyte Bidens laevis possesses suitable cytological characteristics for genotoxicity testing. To test its sensitivity as compared to terrestrial plants species currently in use in standardized assays, Methyl Methanesulfonate (MMS), N-ethyl-N-nitrosourea (ENU) and Maleic Hydrazide (HM) were used. On the other hand, the insecticide Endosulfan (ES) an environmentally relevant contaminant was assayed in seeds and two-month old plants. Mitotic Index (MI), frequency of Chromosome Aberrations in AnaphaseeTelophase (CAAT) and frequency of Abnormal Metaphases (AM) were analyzed. MH, MMS and ENU caused a significant decrease of the MI. MMS was aneugenic whereas MH and ENU were both aneugenic and clastogenic. ES caused a significant concentration-dependent increase of total and aneugenic-CAAT in roots and a significant high frequency of AM at high concentrations. Because of its sensitivity to mutagenic substances, B. laevis can be regarded as a reliable and convenient species for genotoxicity assays especially if aquatic contaminants are evaluated.