LIGHT DEPENDENT PROTEIN PHOSPHORYLATION INDEPENDENT OF PHOTORECEPTORS IN ARABIDOPSIS

LUCIANA CASTRO; ARICO DENISE; MAZZELLA MA

Congreso; RAFV Reunion Argentina de Fisiologia Vegetal; 2018

Light is an environmental factor that regulates plant development. It triggers a signaling cascade that activates genes involved in the change from scotomorphogenesis to photomorphogenesis, some of which are mediated by a process of phosphorylation-dephosphorylation. In order to study the Arabidopsis thaliana phosphoproteome induced by light, a large scale phosphoproteomic study was made from which we selected four phosphoproteins, from a total of thirty six, that changed their phosphorylation status independently of phytochrome and cryptochrome activation. One of these proteins is an alternative splicing factor, another one is a transcription factor involved in abiotic stress and the other two are eukaryotic initiation factors involved in the initiation phase of translation. Preliminary results observed in transcient expression experiments suggests that the alternative splicing factor localized in the nuclei can cause severe damage in Nicotiana benthamiana leaves. We have obtained stable transformants in Arabidopsis thaliana and also mutants and we are currently studying their phosphorilation patterns and their biological function in light signaling