HISTAMINE H2 RECEPTOR AGONIST AMTHAMINE MODULATES CELL PROLIFERATION AND MIGRATION VIA ERK1/2 AND SRC PHOSPHORYLATION IN PANCREATIC CANCER CELLS

NORA A MOHAMAD; TAMARA E. GALARZA; GRACIELA P CRICCO; GABRIELA A MARTIN

Congreso; Reunión de Sociedades de Biociencia 2020; 2020

SAIC-SAI-SAFIS

We have previously determined that histamine dose-dependently modulates cell proliferation, metalloproteases activity and migration in the human pancreatic cancer cells PANC-1 and BxPC3. In vivo, the histamine H2 receptor (H2R) antagonist ranitidine hindered the growth of PANC-1 and BxPC3 grafts and the development of PANC-1 lung metastasis in nude mice. The aim of this work was to evaluate the effect of the H2R agonist amthamine on proliferation, gelatinolytic activity and migration in PANC-1 and BxPC3 cells. Results from dose-response curves showed that 100 nM amthamine in PANC-1 and 10 µM in BxPC3 increased the number of colonies by the clonogenic assay (p