Involvement of phospho-Src and TGF-beta type I receptor in the enhancement of mesenchymal features induced in breast cancer cells by conditioned media from normal mammary cells.

GUADALUPE VEDOYA, TAMARA GALARZA, NORA MOHAMAD, GRACIELA CRICCO, GABRIELA MARTIN

Congreso; LXIII Reunión Científica Anual de la Sociedad Argentina de Investigación Clínica; 2018

onventional radiotherapy for breast cancer is delivered in fractionated doses of 2 Gy post-mastectomy or post conservation surgery. We have previously demonstrated that 2 Gy irradiation of MDA-MB-231 and MCF-7 tumor cells produces epithelial-mesenchymal transition (EMT) related changes in surviving cells, through a signaling path that involves Src phosphorylation/activation. We also found that conditioned media from normal mammary MCF-10A cells irradiated or not (2GyCM or CM) increase mesenchymal characteristics in both irradiated (I) and non-irradiated (NI) tumor cell lines.In this work, we investigated whether Src could be involved in the increase of mesenchymal characteristics induced by CM and 2GyCM in MDA-MB-231 and MCF-7 cells (I or NI). Our results showed that CM and 2GyCM increased phospho-Src levels determined by Western blot in both I and NI tumor cell lines (p