Histamine Modulates Cellular Events Involved in Tumor Invasiveness in Pancreatic Carcinoma Cells.

CRICCO G; NÚÑEZ M; MEDINA V; GARBARINO G; MOHAMAD N; GUTIERREZ A; COCCA C; KIRCHHEIMER C; BERGOC R; RIVERA E; MARTIN G

Bled, Slovenia

Congreso; European Histamine Research Society. XXXIV Annual Meeting; 2005

European Histamine Research Society

Progression of metastatic epithelial tumors is characterized by an increased  motility, a decrease in cellular adhesion, high expression and activation of metalloproteases (MMP) and high angiogenesis capability. In the human pancreatic carcinoma cell line PANC-1, histamine (HA) acts as a growth factor modulating cellular proliferation via H1 and H2 histamine receptors. The objective of this work was to study HA action on cellular adhesion and migration and the expression of MMPs in this cell line. Methylene blue staining of formaline fixed cells, between 30 and 60 minutes after seeding, was performed for studying cellular adhesion. Cells had been previously treated with HA for 48 hours. A decrease in the adhesion to plastic was seen at any tested time. At 90 minutes: Control 100%, 10 mM HA = 63%, 10 nM HA = 44%, ANOVA p