Histamine modulates cell growth reactive-oxygen species production and activity of antioxidants enzymes in human cell lines.

MEDINA V; CRICCO G; GARBARINO G; NÚÑEZ M; MARTIN G; COCCA C; BERGOC R; RIVERA E

Iguazú, Argentina

Congreso; XL Reunión de la Sociedad Argentina de Investigaciones Bioquímicas; 2004

SAIB

In the present work  we investigated the capacity of Histamine (HA) to modulate cell proliferation and ROS production as well as the receptors involved in this response. We employed mammary normal (HBL-100) and malignant cell lines (MDA-MB-231, MCF-7). The levels of the free radicals superoxide (O2·-) and hydrogen peroxide (H2O2) and intracellular HA content were determined by flow citometry. The activity of antioxidant enzymes, superoxide dismutase (SOD) and catalase (CAT) were determined by kinetic spectrophotometric techniques. Cell proliferation was evaluated by the clonogenic assay. Cell lines presented intracellular levels of HA directly correlated with their malignancy. In MDA-MB-231, HA via H2R increased camp levels (EC50 0.45 mm) producing in turn a significant inhibition on cell proliferation (p