Histamine is a selective protector against cellular damage produced by ionizing radiation

MEDINA V; CRICCO G; MOHAMAD N; CROCI M; NÚÑEZ M; MARTÍN G; COCCA C; BERGOC R; RIVERA E

Dusseldorf/Kohln, Germany

Congreso; European Histamine Research Society. XXXIII Annual Meeting,; 2004

European Histamine Research Society.

   The present work investigated the capacity of HA to modulate the oxidative damaged produced by ionizing radiation.    In order to study the effect of histamine (HA) on radiosensitivity of transformed cells, MDA-231 (human breast carcinoma cells) were irradiated in vitro with doses ranging from 0 to10 Gy employing a 137Cs source of 189 TBq (Dose rate: 7.7 Gy/min). HA from 0.1 to 10 mM was added to cultures 20 hs before irradiation. The survival curves were adjusted using the Lineal-quadratic model. The levels of the free radicals superoxide (O2·-) and hydrogen peroxide (H2O2) were determined by flow citometry employing the specific fluorescence dyes dichloride fluorescein diacetato and dihydroethidium. In addition the activity of antioxidant enzymes, superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (Gpx) were determined in MDA cells treated with HA 10 mM and/or irradiated with 2Gy.    HA treatment produced a significant dose dependent decrease in cell survival (p poster