Phospho-Src protein mediates radio-induced mesenchymal changes in MCF-7 cells. Modulation by histamine

T. GALARZA, M. TÁQUEZ DELGADO, N. MOHAMAD, G.VEDOYA, V. MEDINA, G. MARTÍN, G CRICCO.

Congreso; European Histamine Research Society 45th Annual Meeting; 2016

European Histamine Research Society

Radiotherapy is a prime option for treatment of solid tumors though an increase in invasiveness of neoplastic cells has been described and associated to the epithelial to mesenchymal transition (EMT) induction. Our previous findings show that histamine may reduce the manifestation of mesenchymal features in irradiated MCF-7 and MDA-MB-231 breast cancer cells. We also determined that phosphorylation/activation of Src kinase is involved in histamine modulated MDA-MB-231 cell migration and invasion.The aim of this work was to investigate the role of phospho-Src (P-Src) in irradiated MCF-7 cells in relation to the invasive phenotype when cells were previously treated with histamine.Cells were treated with 20 µM histamine, 10 µM PP2 (Src inhibitor) or histamine+PP2 for 24 h, and then were irradiated with a dose of 2 Gray which is comparable to that used in fractionation radiotherapy. P-Src levels were determined immediately after irradiation while experiments to evaluate EMT molecular markers and cell migration were performed five days post-irradiation.Immunoblots show that ionizing radiation produced an increase in P-Src protein level (p